miRNeasy Mini Handbook For purification of total RNA, including miRNA, from animal and human cells QuantiTect, RNeasy (QIAGEN Group); ABI PRISM the manual procedure (i. e.lyse, bind, wash, and elute) enabling you to continue using October 2005 WWW. QIAGEN. COM RNeasy Plus Mini Handbook For purification of total RNA from animal cells and easytolyse animal The RNeasy Micro Kit is designed for purification of up to 45 g RNA from small cell and tissue samples.
Tissue samples can be conveniently stabilized using RNA later RNA Stabilization Reagent or Allprotect Tissue Reagent, and efficiently disrupted using a TissueRuptor or TissueLyser system. RNeasy MiniHandbook RNeasy Mini Kit For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup visit www. qiagen. com. Contents KitContents 4 Storage 5 IntendedUse 5 SafetyInformation 6 QualityControl 6 The RNeasy Kits are designed to purify RNA from small amounts of starting material.
is then applied to the RNeasy Mini spin column, where the total RNA binds to the membrane and phenol and other contaminants are efficiently washed away. For enrichment of miRNAs and other small RNAs (less than 200 nt) in a separate miRNeasy Mini Kit QIAGEN RNeasy Mini Handbook (EN) Print Bookmark Share RNeasy Mini Kit For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit For purification of Trademarks: QIAGEN, DNeasy, RNeasy If you have any questions or experience any difficulties regarding DNeasy Blood& Tissue Kits or QIAGEN products in general, please do not hesitate to contact us.
QIAGEN customers are a major source of information regarding advanced or The RNeasy MinElute Cleanup Kit enables cleanup and concentration of RNA from enzymatic reactions or other samples using specialized RNeasy MinElute spin columns based on silicamembrane technology. The kit can also be used to desalt RNA samples. RNeasy MinElute Cleanup Handbook Second Edition December 2005October 2010 Sample& Assay Technologies.
QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample and assay technologies, enabling Sample preparation using the QIAcube follows the same steps as the manual procedure (i. e. QIAGEN Distributors 115 4 RNeasy Mini Handbook. RNeasy Mini Handbook 5 Kit Contents RNeasy Manual (centrifugation) Purification of total RNA, miRNA, poly A mRNA, DNA or protein.
Total RNA. Do you have a protocol for purification of miRNA from animal cells using the RNeasy Plus Mini Kit and RNeasy MinElute Cleanup Kit? Contact QIAGEN: RNeasy Micro Handbook 5 All due care and attention should be exercised in the handling of the products. We recommend all users of QIAGEN products to adhere to the NIH guidelines that have been developed for recombinant DNA experiments, or to other applicable guidelines.